RECIPROCAL REGULATION OF PREPROTHYROTROPIN-RELEASING HORMONE (TRH) MESSENGER-RNA IN THE RAT ANTERIOR HYPOTHALAMUS BY THYROID-HORMONE - DISSOCIATION FROM TRH CONCENTRATIONS DURING HYPOTHYROIDISM

被引:20
作者
YAMADA, M [1 ]
WILBER, JF [1 ]
机构
[1] LOUISIANA STATE UNIV,MED CTR,ENDOCRINOL & METAB SECT,1542 TULANE AVE,NEW ORLEANS,LA 70112
关键词
D O I
10.1016/0143-4179(90)90160-Z
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
TRH mRNA has not been quantified concomitantly with TRH itself to examine graded effects of thyroid hormones (TH) upon TRH gene transcriptional regulation and post-transcriptional expression. To examine such TH effects, rats were rendered thyrotoxic with L-T3 (50 μg per 100 g body weight) or hypothyroid by total thyroidectomy. After decapitation, frozen coronal brain sections were prepared in a matrix for hypothalamic micropunches. PreProTRH mRNA was quantified in punch pools by slot - blot hybridization and densitometry, using a 32P 1.2 Kb rat riboprobe (gift of Drs. S. Lee and R. Goodman). T3 toxicosis resulted in parallel reductions in PreProTRH mRNA (-45%) and TRH concentrations determined by RIA (-43%), 1.1 versus 1.9 ng/mg protein, P < 0.01. Conversely, elevations in PreProTRH mRNA were stimulated by hypothyroidism on Day 14 (+32%). However, TRH concentrations were reduced paradoxically from 2.2 ± 0.05 ng/mg protein to 0.68 ± 0.03, p < 0.01. No changes, in contrast, were identified in whole hypothalamic extracts in either PreProTRH mRNA or TRH after T3 treatment. It is concluded that TH do exert inhibitory effects upon PreProTRH mRNA transcription, and in the specific hypothalamic nucleus concerned with thyroid regulation (PVN). The failure of TRH concentrations to rise concordantly with activated TRH gene transcription in hypothyroidism suggests that TH may exert inhibitory effects upon PreProTRH mRNA translation, in addition to inhibition of TRH gene transcription and TRH secretion. © 1990.
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页码:49 / 53
页数:5
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