MOLECULAR-SIZE OF THE RENAL SODIUM-PHOSPHATE SYMPORTER IN NATIVE AND RECONSTITUTED SYSTEMS

被引:15
作者
DELISLE, MC
VACHON, V
GIROUX, S
POTIER, M
LAPRADE, R
BELIVEAU, R
机构
[1] UNIV MONTREAL, RECH TRANSPORT MEMBRANAIRE GRP, MONTREAL H3C 3J7, QUEBEC, CANADA
[2] UNIV MONTREAL, HOP ST JUSTINE, SERV GENET MED, MONTREAL H3T 1C5, QUEBEC, CANADA
基金
加拿大自然科学与工程研究理事会; 英国医学研究理事会;
关键词
RECONSTITUTION; MOLECULAR SIZE; PHOSPHATE TRANSPORT; SODIUM ION SYMPORT; KIDNEY; (BOVINE);
D O I
10.1016/0005-2736(92)90141-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The size of the renal sodium/phosphate symporter was estimated with the radiation inactivation technique in isolated bovine brush border membrane vesicles and after reconstitution in proteoliposomes. The functional unit of the native phosphate carrier had a radiation inactivation size of 172 +/- 17 kDa. Identical values were obtained for the reconstituted carrier whether it was irradiated before or after the formation of the proteoliposomes (161 +/- 9 and 159 +/- 11 kDa, respectively). The sodium-independent uptake of phosphate was not affected significantly by radiation doses up to 10 Mrad. This activity is therefore not due to the reconstitution of a large phosphate-binding protein such as alkaline phosphatase. Furthermore, bromotetramisole, a specific inhibitor of phosphate binding to this enzyme, had no significant effect on the uptake of phosphate by the proteoliposomes.
引用
收藏
页码:132 / 136
页数:5
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