UV-INDUCED G-C-]A-T TRANSITIONS AT THE APRT LOCUS OF CHINESE-HAMSTER OVARY CELLS CLUSTER AT FREQUENTLY DAMAGED 5'-TCC-3' SEQUENCES

被引：19

作者：

DROBETSKY, EA ^{[1
]}

SAGE, E ^{[1
]}

机构：

[1] INST CURIE,BIOL SECT,CNRS,URA 1292,F-75231 PARIS 05,FRANCE

来源：

MUTATION RESEARCH | 1993年 / 289卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

MUTATIONAL SPECIFICITY; DAMAGE DISTRIBUTION; ULTRAVIOLET LIGHT; DNA CONTEXT; CYCLOBUTANE PYRIMIDINE DIMERS; (6-4) PYRIMIDINE PYRIMIDONE PHOTOPRODUCT;

D O I：

10.1016/0027-5107(93)90062-K

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have determined the relative frequency in vitro of UV-induced cyclobutane pyrimidine dimers (py < > py) and (6-4) pyrimidine pyrimidone photoproducts (py(6-4)pyo) at individual sites in selected regions of the Chinese hamster ovary (CHO) adenine phosphoribosyltransferase (aprt) gene, and compared this to the observed specificity of UV-induced mutations (Drobetsky et al., 1987, 1989). Our results indicate that G: C --> A: T transition '' hotspots'' (multiple occurrences) at the chromosomal CHO aprt locus, the majority of which occur at 5'-TCC-3', are clearly targeted at sites associated with a relatively high yield of py < > py and/or py(6-4)pyo. We conclude that photoproduct frequency plays a major role in UV-induced transition mutagenesis at 5'-TCC-3' sites at an endogenous locus in a rodent cell line, and that both py(6-4)pyo and py < > py have premutagenic potential.

引用

页码：131 / 138

页数：8

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