SIGNIFICANCE OF PH ON DIFFERENTIATION OF HUMAN ERYTHROID CELL-LINES

被引：8

作者：

ENDO, T

ISHIBASHI, Y

OKANA, H

FUKUMAKI, Y

机构：

[1] KYUSHU UNIV,FAC DENT,DEPT ORAL PATHOL,FUKUOKA,FUKUOKA,JAPAN

[2] KINKI UNIV,KYUSHU JR COLL,OSAKA,OSAKA,JAPAN

[3] KYUSHU UNIV,GENET INFORMAT RES LAB,FUKUOKA,FUKUOKA,JAPAN

来源：

LEUKEMIA RESEARCH | 1994年 / 18卷 / 01期

关键词：

ERYTHROID CELL LINE; KU-812; K562; SPONTANEOUS DIFFERENTIATION; HIGH PH MEDIUM; BOVINE SERUM ALBUMIN; HEMIN; ISOELECTRIC FOCUSING;

D O I：

10.1016/0145-2126(94)90008-6

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

As physiological factors and compounds, BSA and/or higher pH of the culture medium could induce the erythroid differentiation of cells. Optimum pH values of the culture medium for higher spontaneous differentiation of KU-812 and K562 cells after 7 days cultivation were 7.5 and 7.6, respectively. The synergistic effects on the differentiation were observed by exposure to hemin under the higher pH condition. In the presence of BSA, 25% of KU-812 and 40% of K562 cells became benzidine positive. Synergistic effects of BSA and the higher pH of the medium were also observed.

引用

页码：49 / 54

页数：6

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