DIFFERENT TARGET-SITE SPECIFICITIES OF THE HAIRPIN RIBOZYME IN CIS AND TRANS CLEAVAGES

被引：5

作者：

FUJITANI, K ^{[1
]}

SASAKITOZAWA, N ^{[1
]}

KIKUCHI, Y ^{[1
]}

机构：

[1] MITSUBISHI KASEI INST LIFE SCI, 11 MINAMIOOYA, MACHIDA, TOKYO 194, JAPAN

来源：

FEBS LETTERS | 1993年 / 331卷 / 1-2期

关键词：

CATALYTIC RNA; SATELLITE RNA; TOBACCO RINGSPOT VIRUS; ARABIS MOSAIC VIRUS; CHICORY YELLOW MOTTLE VIRUS;

D O I：

10.1016/0014-5793(93)80316-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The hairpin ribozyme cleaves a phosphodiester bond at the 5' side of a 5'GUC3' sequence of an RNA with high efficiency. An RNA having a 5'GUA3' sequence instead of the GUC sequence is a poor substrate for this ribozyme. Here, we show that this is indeed so in a trans-acting ribozyme system, but in a cis-acting ribozyme system this ribozyme cleaves the 5' side of a GUA sequence as efficiently as the wild-type cleaves the GUC sequence. One base substitution in the ribozyme also affected the target-site specificity in the cis-acting system.

引用

页码：155 / 158

页数：4

共 15 条

[1] IMAGING PLATE ILLUMINATES MANY FIELDS [J].

AMEMIYA, Y ;

MIYAHARA, J .

NATURE, 1988, 336 (6194) :89-90

[2] ESSENTIAL NUCLEOTIDE-SEQUENCES AND SECONDARY STRUCTURE ELEMENTS OF THE HAIRPIN RIBOZYME [J].

BERZALHERRANZ, A ;

JOSEPH, S ;

CHOWRIRA, BM ;

BUTCHER, SE ;

BURKE, JM .

EMBO JOURNAL, 1993, 12 (06) :2567-2574

[3]

BRUENING G, 1989, METHOD ENZYMOL, V180, P546

[4] NOVEL GUANOSINE REQUIREMENT FOR CATALYSIS BY THE HAIRPIN RIBOZYME [J].

CHOWRIRA, BM ;

BERZALHERRANZ, A ;

BURKE, JM .

NATURE, 1991, 354 (6351) :320-322

[5] SPECIFIC ASSOCIATION BETWEEN AN ENDORIBONUCLEOLYTIC SEQUENCE FROM A SATELLITE RNA AND A SUBSTRATE-ANALOG CONTAINING A 2'-5' PHOSPHODIESTER [J].

FELDSTEIN, PA ;

BUZAYAN, JM ;

VANTOL, H ;

DEBEAR, J ;

GOUGH, GR ;

GILHAM, PT ;

BRUENING, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (07) :2623-2627

[6] 2 SEQUENCES PARTICIPATING IN THE AUTOLYTIC PROCESSING OF SATELLITE TOBACCO RINGSPOT VIRUS COMPLEMENTARY RNA [J].

FELDSTEIN, PA ;

BUZAYAN, JM ;

BRUENING, G .

GENE, 1989, 82 (01) :53-61

[7] SELF-CLEAVAGE OF PLUS AND MINUS RNAS OF A VIRUSOID AND A STRUCTURAL MODEL FOR THE ACTIVE-SITES [J].

FORSTER, AC ;

SYMONS, RH .

CELL, 1987, 49 (02) :211-220

[8] HAIRPIN CATALYTIC RNA MODEL - EVIDENCE FOR HELICES AND SEQUENCE REQUIREMENT FOR SUBSTRATE RNA [J].

HAMPEL, A ;

TRITZ, R ;

HICKS, M ;

CRUZ, P .

NUCLEIC ACIDS RESEARCH, 1990, 18 (02) :299-304

[9] RNA CATALYTIC PROPERTIES OF THE MINIMUM (-)STRSV SEQUENCE [J].

HAMPEL, A ;

TRITZ, R .

BIOCHEMISTRY, 1989, 28 (12) :4929-4933

[10] SEQUENCES REQUIRED FOR SELF-CATALYZED CLEAVAGE OF THE SATELLITE RNA OF TOBACCO RINGSPOT VIRUS [J].

HASELOFF, J ;

GERLACH, WL .

GENE, 1989, 82 (01) :43-52

← 1 2 →