SANDWICH CAPTURE ELISA BY A MURINE MONOCLONAL-ANTIBODY AGAINST A GENUS-SPECIFIC LPS EPITOPE FOR THE DETECTION OF DIFFERENT COMMON SEROTYPES OF SALMONELLAS

被引:35
作者
CHOI, D [1 ]
TSANG, RSW [1 ]
NG, MH [1 ]
机构
[1] UNIV HONG KONG,DEPT MICROBIOL,PATHOL BLDG,QUEEN MARY HOSP COMPOUND,HONG KONG,HONG KONG
来源
JOURNAL OF APPLIED BACTERIOLOGY | 1992年 / 72卷 / 02期
关键词
D O I
10.1111/j.1365-2672.1992.tb01814.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A sandwich capture ELISA based on a murine monoclonal antibody against a genus-specific epitope in the outer core region of the Salmonella lipopolysaccharide is described for the detection of different common serotypes of salmonellas. Four h broth cultures of seven standard and 24 wild strains of salmonellas were all detected by the capture ELISA while overnight broth cultures of 21 non-salmonella standard strains were all negative. The capture ELISA detected 1 ng/ml of Ra lipopolysaccharide, 10(6)/ml of a smooth wild strain of Salm. typhimurium, and 1120 cells of Salm. heidelberg after enrichment culture for 4 h.
引用
收藏
页码:134 / 138
页数:5
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