3 TANDEMLY REPEATED 5S RIBOSOMAL-RNA-ENCODING GENES IDENTIFIED, CLONED AND CHARACTERIZED FROM CRYPTOSPORIDIUM-PARVUM

被引：10

作者：

TAGHIKILANI, R

REMACHAMORENO, M

WENMAN, WM

机构：

[1] UNIV ALBERTA,DEPT PEDIAT,DIV INFECT DIS,EDMONTON T6G 2R7,AB,CANADA

[2] UNIV ALBERTA HOSP,PROV LAB PUBL HLTH,EDMONTON T6G 2R7,AB,CANADA

[3] UNIV ALBERTA HOSP,DEPT MICROBIOL & INFECT DIS,EDMONTON T6G 2R7,AB,CANADA

来源：

GENE | 1994年 / 142卷 / 02期

关键词：

PROTOZOAN PARASITE; RIBOSOMAL-RNA GENE; 5S; OOCYST; PCR; REPEAT; POLYMERASE III;

D O I：

10.1016/0378-1119(94)90270-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have characterized the 5S rRNA of Cryptosporidium parvum. The gene (rDNA) encoding this 5S rRNA was identified, mapped, the primary and secondary structures determined, and the copy number estimated. Using a PCR-amplified 5S rDNA as a probe, it was shown that this gene can specifically recognize C. parvum genomic DNA, but not other intestinal and environmental organisms tested. Three repeat units of the 5S rDNA found in genomic C. parvum oocyst DNA are within the 2012-bp EcoRI-HindIII fragment and are identical in coding sequence, but differ in flanking regions. Flanking regions are A + T rich (78-89%). The termination signal for polymerase III consists of five thymidine residues at the 3' end of each of three units.

引用

页码：253 / 258

页数：6

共 16 条

[1] NUCLEOTIDE-SEQUENCES IN XENOPUS 5S DNA REQUIRED FOR TRANSCRIPTION TERMINATION [J].