EFFECT OF METHYL-METHACRYLATE ON MITOCHONDRIAL-FUNCTION AND STRUCTURE

被引:15
作者
BEREZNOWSKI, Z [1 ]
机构
[1] ACAD MED SCH GDANSK,DEPT BIOCHEM,PL-80208 GDANSK,POLAND
来源
INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1994年 / 26卷 / 09期
关键词
D O I
10.1016/0020-711X(94)90134-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
1. Treatment of isolated rat liver mitochondria with methyl methacrylate (MM) produced membrane disruption as evidenced by the release of citrate synthase, and changes in the ultrastructure of mitochondria. 2. At concentration 0.1%, MM uncoupled oxidative phosphorylation as evidenced by stimulation of state 4 respiration supported either by pyruvate plus malate or succinate (+rotenone) and ATP-ase activity in intact mitochondria. 3. At concentration 1% MM stimulated ATP-ase activity in intact mitochondria and succinate (+ rotenone) oxidation at state 4 and was without effect on this substrate oxidation at state 3. 4. MM inhibited pyruvate plus malate oxidation either at state 3 or in the presence of uncoupling agents. 5. MM inhibited the NADH oxidase of electron transport particles at a concentration which failed to inhibit either succinic oxidase or the NADH-ferricyanide reductase activity. 6. The data presented suggest that in the isolated mitochondria MM inhibits NADH oxidation in the vicinity of the rotenone sensitive site of complex I. 7. The general conclusion is that MM may block an electron transport and to uncouple oxidative phosphorylation in rat liver mitochondria. The overall in vitro effect would be to prevent ATP synthesis which could result in cell death under in vivo conditions.
引用
收藏
页码:1119 / 1127
页数:9
相关论文
共 37 条