ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR DETECTION OF POTATO VIRUSES A AND Y IN POTATO LEAVES AND SPROUTS

被引：30

作者：

MAAT, DZ

DEBOKX, JA

机构：

[1] Instituut voor Plantenziektenkundig Onderzoek, Wageningen, 6700 AA

来源：

NETHERLANDS JOURNAL OF PLANT PATHOLOGY | 1978年 / 84卷 / 05期

关键词：

D O I：

10.1007/BF02650382

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

With the enzyme-linked immunosorbent assay (ELISA) potato virus A (PVA) could be detected reliably in potato sprouts, especially when these were young and sappy. The detection of this virus in leaves of glasshouse-grown potato plants was less reliable. The tobacco veinal necrosis strain of potato virus Y (PVYN) was readily demonstrated in foliage of glass-house-grown potato plants using an antiserum to this strain. Plants infected with the common strain (PVYO) did not react in ELISA with this antiserum. In young sappy sprouts, using the PVYN antiserum, PVYN could be detected reliably when samples with PVYO were excluded, as the reaction of samples infected with the latter virus was intermediate between PVYN-diseased and PVY-free samples. PVY was also detected in plants inadvertently infected during the experiments. © 1978 Kluwer Academic Publishers.

引用

页码：167 / 173

页数：7

共 7 条

[1] de Bokx J.A., Test plants, Viruses of potatoes and seed-potato production, pp. 102-110, (1972)
[2] de Bokx J.A., Kratchanova B., Maat D.Z., Some properties of a deviating strain of potato virus Y, Potato Res., 18, pp. 38-51, (1975)
[3] Clark M.F., Ada A.N., Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses, J. gen. Virol., 34, pp. 475-483, (1977)
[4] Casper R., Detection of potato leafroll virus in potato and in Physalis floridana by enzyme-linked immunosorbent assay (ELISA), Journal of Phytopathology, 90, pp. 364-368, (1977)
[5] Maat D.Z., Mierzwa Z., Potato virus A: purification and antiserum preparation, Potato Res., 18, pp. 126-129, (1975)
[6] Maat D.Z., de Bokx J.A., Potato leafroll virus: antiserum preparation and detection in potato leaves and sprouts with the enzyme-linked immunosorbent assay (ELISA), Neth. J. Pl. Path., 84, pp. 149-156, (1978)
[7] Steinbuch M., Audran R., The isolation of IgG from mammalian sera with the aid of caprylic acid, Arch. Biochem. Biophys., 134, pp. 279-284, (1969)

← 1 →