ISOLATION AND PARTIAL CHARACTERIZATION OF A THIOL PROTEINASE-INHIBITOR FROM HUMAN-PLASMA

被引：63

作者：

RYLEY, HC

机构：

[1] Department of Medical Microbiology, Welsh National School of Medicine, Cardiff, CF4 4XN Wales, Health Park

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 89卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)91859-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A thiol proteinase inhibitor has been isolated from human plasma by ion exchange, salt-mediated hydrophobic and ion chelation chromatography. It was found to be electrophoretically heterogeneous (in both its native state and after isolation) giving a bimodal arc with an α1 and α2 peak in bidimensional immunoelectrophoresis. It was a good inhibitor of papain but only partially inhibited human kidney cathepsin Bl and did not inhibit the bacterial thiol proteinase, clostripain. Its mean protein concentration in adults sera was 42.5 ± 6.8 mg per dl. © 1979.

引用

页码：871 / 878

页数：8

共 17 条

[1] METHODS FOR QUANTITATIVE ESTIMATION OF N-ACETYLNEURAMINIC ACID AND THEIR APPLICATION TO HYDROLYSATES OF SIALOMUCOIDS [J].