RIBONUCLEASE-ACTIVITIES IN DEVELOPING EXPERIMENTAL GRANULATION-TISSUE WITH REFERENCE TO POLYSOMES

The Mg2+‐precipitable polysomes in experimental granulation tissue increase up to three weeks and decrease slowly thereafter. Polysomes from young granulation tissue are, on a weight basis, more active in cell‐free protein synthesis than polysomes from old granulation tissue. RNase activity is highest in polysomes from 1‐week tissue. The polysomes contain both acid and alkaline RNase, but the alkaline RNase is in latent form, manifested by the addition of p‐chloromercuribenzoate (pCMB). The high RNase activity of granulation tissue renders the analysis of polysomal patterns and functions of the polysomes difficult. In the post‐polysomal supernatant of granulation tissue the RNase activity is highest in 2–4 week old tissue; it is maximal at pH 7–8 and only slightly influenced by pCMB. © 1979 Scandinavian Physiological Society