THERMODYNAMIC STABILITY AND DRUG-BINDING PROPERTIES OF OLIGODEOXYRIBONUCLEOTIDE DUPLEXES CONTAINING 3-DEAZAADENINE - THYMINE BASE-PAIRS

被引：11

作者：

LEVER, C

LI, X

COSSTICK, R

EBEL, S

BROWN, T

机构：

[1] UNIV LIVERPOOL,ROBERT ROBINSON LABS,DEPT CHEM,POB 147,LIVERPOOL L69 3BX,ENGLAND

[2] EDINBURGH CTR MOLEC RECOGNIT,DEPT CHEM,EDINBURGH EH9 3JJ,SCOTLAND

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 08期

关键词：

D O I：

10.1093/nar/21.8.1743

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used ultraviolet melting techniques to study the effect on stability of incorporating the nucleoside analogue 2'-deoxy-3-deazaadenosine (d(3C)A) into the duplex 5'-d(CGCAATCG)-3'-d(GCGTTAGC). Our results demonstrate that the successive replacement of dA by d(3C)A increasingly destabilises the duplex. The destabilising effect of this analogue is considerably enhanced as the pH is lowered and the results are consistent with protonation of 3-deazaadenine (probably at N-1) contributing to duplex destabilisation. Surprisingly, the incorporation of d(3C)A does not significantly affect the binding of distamycin-A.

引用

页码：1743 / 1746

页数：4

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