A PRACTICAL APPROACH TO CROSS-LINKING

被引：224

作者：

MATTSON, G

CONKLIN, E

DESAI, S

NIELANDER, G

SAVAGE, MD

MORGENSEN, S

机构：

[1] Technical Assistance Department, Pierce Chemical Company, Rockford, 61105, IL

来源：

MOLECULAR BIOLOGY REPORTS | 1993年 / 17卷 / 03期

关键词：

CARBODIIMIDE; CROSS-LINKERS; HOMOBIFUNCTIONAL; HETEROBIFUNCTIONAL; IMIDO ESTERS; MALEIMIDE; NHS ESTERS; PHENYL AZIDE; PYRIDYL DISULFIDE;

D O I：

10.1007/BF00986726

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The various aspects of chemical crosslinking are addressed. Crosslinker reactivity, specificity, spacer arm length and solubility characteristics are detailed. Considerations for choosing one of these crosslinkers for a particular application are given as well as reaction conditions and practical tips for use of each category of crosslinkers.

引用

页码：167 / 183

页数：17

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