FORMATION OF 22-CARBON AND 24-CARBON 6-DESATURATED FATTY-ACIDS FROM EXOGENOUS DEUTERATED ARACHIDONIC-ACID IS ACTIVATED IN THP-1 CELLS AL HIGH SUBSTRATE CONCENTRATIONS

被引：21

作者：

CARUSO, D ^{[1
]}

RISE, P ^{[1
]}

GALELLA, G ^{[1
]}

REGAZZONI, C ^{[1
]}

TOIA, A ^{[1
]}

GALLI, G ^{[1
]}

GALLI, C ^{[1
]}

机构：

[1] UNIV MILAN,INST PHARMACOL SCI,I-20093 MILAN,ITALY

来源：

FEBS LETTERS | 1994年 / 343卷 / 03期

关键词：

THP-1; CELL; DEUTERATED; 20; 4; N-6; GC-MS; 6 DESATURATION OF 24; 4 DESATURATION IN 22; 5;

D O I：

10.1016/0014-5793(94)80554-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Deuterated arachidonic acid (AA, [H-2(8)]20:4 n-6) 1-25 mu M, is converted to other fatty acids, as evaluated by gas chromatography-mass spectrometry, in THP-1 cells. The major products, in the 1 to 10 mu M range, are 22:4 (elongated) and 20:3 (reduced in 5). At 25 mu M, 24:4, 24:5 and 22:5 accumulate, with [H-2(8)]/[H-2(0)] ratios higher than in AA. At high AA concentration preferential conversion to elongated fatty acids with 5 unsaturations, through a 6 desaturase takes place and the 4-desaturated 22:5 appears to be formed through B-oxidation of 24:5.

引用

页码：195 / 199

页数：5

共 16 条

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