ISOLATION AND CHARACTERIZATION OF A GENE FROM TRYPANOSOMA-CRUZI ENCODING A 46-KILODALTON PROTEIN WITH HOMOLOGY TO HUMAN AND RAT TYROSINE AMINOTRANSFERASE

被引：26

作者：

BONTEMPI, EJ

BUA, J

ASLUND, L

PORCEL, B

SEGURA, EL

HENRIKSSON, J

ORN, A

PETTERSSON, U

RUIZ, AM

机构：

[1] MINIST SALUD & ACC SOCIAL,INST NACL DIAGNOST & INVEST ENFERMEDAD CHAGAS,AVE PASEO COLON 568,RA-1063 BUENOS AIRES,ARGENTINA

[2] KAROLINSKA INST,DEPT IMMUNOL,S-10401 STOCKHOLM 60,SWEDEN

[3] UNIV UPPSALA,CTR BIOMED,DEPT MED GENET,S-75105 UPPSALA,SWEDEN

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1993年 / 59卷 / 02期

关键词：

TRYPANOSOMA-CRUZI; CHAGAS DISEASE; TYROSINE AMINOTRANSFERASE; DNA SEQUENCE; TANDEMLY REPEATED GENES; CHROMOSOMAL LOCALIZATION;

D O I：

10.1016/0166-6851(93)90223-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complete sequence of a gene encoding a 46-kDa protein of Trypanosoma cruzi is presented. The first ATG complies with the consensus sequence for initiation of translation. A single band of 2 kb was highlighted by hybridizing a pro the 46-kDa protein gene to a Northern filter containing total T. cruzi RNA. The gene is present in 50-80 copies per cell and most of them are contained in 2 tandem arrays on large T. cruzi chromosomes ( > 2000 kb). A strong homology with rat and human tyrosine aminotransferase was detected. Homology with a Trypanosoma brucei retrotransposon was found in the nonsense strand of the intergenic region.

引用

页码：253 / 262

页数：10

共 37 条

[1] TRYPANOSOMA-CRUZI - CELLULAR AND ANTIBODY-RESPONSE AGAINST THE PARASITE IN MICE IMMUNIZED WITH A 19-AMINO ACID SYNTHETIC PEPTIDE [J].

BUA, J ;

BONTEMPI, EJ ;

LEVIN, M ;

ORN, A ;

VELASCO, D ;

MORENO, M ;

LEVIYEYATI, P ;

ENGSTROM, A ;

SEGURA, EL ;

RUIZ, AM .

EXPERIMENTAL PARASITOLOGY, 1991, 72 (01) :54-62

[2] THE MAJOR CYSTEINE PROTEINASE (CRUZIPAIN) FROM TRYPANOSOMA-CRUZI IS ENCODED BY MULTIPLE POLYMORPHIC TANDEMLY ORGANIZED GENES LOCATED ON DIFFERENT CHROMOSOMES [J].

CAMPETELLA, O ;

HENRIKSSON, J ;

ASLUND, L ;

FRASCH, ACC ;

PETTERSSON, U ;

CAZZULO, JJ .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1992, 50 (02) :225-234

[3] TRYPANOSOMA-CRUZI RIBOSOMAL-RNA - INTERNAL BREAK IN THE LARGE-MOLECULAR MASS SPECIES AND NUMBER OF GENES [J].

CASTRO, C ;

HERNANDEZ, R ;

CASTANEDA, M .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1981, 2 (3-4) :219-233

[4] GENOME ORGANIZATION AND PLOIDY NUMBER IN TRYPANOSOMA-CRUZI [J].

CASTRO, C ;

CRAIG, SP ;

CASTANEDA, M .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1981, 4 (5-6) :273-282

[5] CHARACTERIZATION OF A CANDIDATE GENE FOR GP72, AN INSECT STAGE-SPECIFIC ANTIGEN OF TRYPANOSOMA-CRUZI [J].

COOPER, R ;

INVERSO, JA ;

ESPINOSA, M ;

NOGUEIRA, N ;

CROSS, GAM .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 49 (01) :45-60

[6] A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].

DEVEREUX, J ;

HAEBERLI, P ;

SMITHIES, O .

NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395

[7] THE GENOME OF TRYPANOSOMA-CRUZI CONTAINS A CONSTITUTIVELY EXPRESSED, TANDEMLY ARRANGED MULTICOPY GENE HOMOLOGOUS TO A MAJOR HEAT-SHOCK PROTEIN [J].

DRAGON, EA ;

SIAS, SR ;

KATO, EA ;

GABE, JD .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (03) :1271-1275

[8]

EICKBUSH TH, 1992, NEW BIOL, V4, P430

[9] TRYPANOSOMA-CRUZI - BIOLOGICAL CHARACTERIZATION OF 19 CLONES DERIVED FROM 2 CHRONIC CHAGASIC PATIENTS .1. GROWTH-KINETICS IN LIQUID-MEDIUM [J].

ENGEL, JC ;

DVORAK, JA ;

SEGURA, EL ;

CRANE, MSJ .

JOURNAL OF PROTOZOOLOGY, 1982, 29 (04) :555-560

[10] COMPARISON OF GENES ENCODING TRYPANOSOMA-CRUZI ANTIGENS [J].

FRASCH, ACC ;

CAZZULO, JJ ;

ASLUND, L ;

PETTERSSON, U .

PARASITOLOGY TODAY, 1991, 7 (06) :148-151

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