2 AFFINITY CHROMATOGRAPHY METHODS FOR PURIFICATION OF GLYOXALASE-I FROM RABBIT LIVER

The purification of Glyoxalase I from rabbit liver using Blue Dextran-Sepharose-4B and S-hexyl Glutathione Sepharose-6B is described. Elution of Glyoxalase I from both the columns was accomplished with S-hexyl glutathione, a competitive inhibitor of the enzyme. The purified enzyme gave two bands on disc electrophoresis. After treatment with glutathione, only one band was found. Except for these interconvertible forms, the purified enzyme was homogeneous as shown by disc electrophoresis and sodium dodecyl sulfate polyacrylamide gel electrophoresis. © 1978.