ALFALFA TRYPSIN-INHIBITOR

被引：16

作者：

CHANG, HY ^{[1
]}

REECK, GR ^{[1
]}

MITCHELL, HL ^{[1
]}

机构：

[1] KANSAS STATE UNIV,DEPT BIOCHEM,MANHATTAN,KS 66506

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1978年 / 26卷 / 06期

关键词：

D O I：

10.1021/jf60220a030

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Trypsin inhibitor was isolated from defatted alfalfa by extracting with 0.2 M NaCl, adsorbing on a trypsin-Sepharose affinity column, and eluting with a glycine buffer. Subsequent CM-cellulose chromatography did not improve its purity appreciably. Its amino acid composition was compared with that of Bowman-Birk soybean trypsin inhibitor and with an average composition of other proteins. Inhibitor content increased as plants matured. The inhibitor is present in greatest concentration in leaves. © 1978, American Chemical Society. All rights reserved.

引用

页码：1463 / 1464

页数：2

共 12 条

[1] PURIFICATION OF A TRYPSIN INHIBITOR OF ALFALFA [J].