HIGH-MOLECULAR-WEIGHT FORMS OF BASIC FIBROBLAST GROWTH-FACTOR RECOGNIZED BY A NEW ANTI-BFGF ANTIBODY

被引：35

作者：

GROTHE, C

ZACHMANN, K

UNSICKER, K

WESTERMANN, R

机构：

[1] Department of Anatomy and Cell Biology, Philipps-University of Marburg, D-3550 Marburg

来源：

FEBS LETTERS | 1990年 / 260卷 / 01期

关键词：

Adrenal; Antibody; Fibroblast growth factor; basic; Ovary; Pituitary;

D O I：

10.1016/0014-5793(90)80059-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An antibody against basic fibroblast growth factor (bFGF) was raised using purified bovine pituitary bFGF. Western blot analysis revealed immunoreactive bands at 18, 24, 30-33 and 46 kDa in inununoaffinity purified extracts of pituitary and adrenal gland using this antibody. A similar staining pattern was obtained with ovary extracts with the exception of the missing 18 kDa band. A second anti-bFGF antibody raised against a synthetic peptide comprising the 24 N-terminal amino acids of bFGF reacted with the 18 kDa and the 46 kDa band of immunoaffinity purified ovary and adrenal gland extracts. © 1990.

引用

页码：35 / 38

页数：4

共 23 条

[1] NUCLEOTIDE-SEQUENCE OF A BOVINE CLONE ENCODING THE ANGIOGENIC PROTEIN, BASIC FIBROBLAST GROWTH-FACTOR
ABRAHAM, JA
MERGIA, A
WHANG, JL
TUMOLO, A
FRIEDMAN, J
HJERRILD, KA
GOSPODAROWICZ, D
FIDDES, JC
[J]. SCIENCE, 1986, 233 (4763) : 545 - 548
[2] BASIC FIBROBLAST GROWTH-FACTOR PREVENTS DEATH OF LESIONED CHOLINERGIC NEURONS INVIVO
ANDERSON, KJ
DAM, D
LEE, S
COTMAN, CW
[J]. NATURE, 1988, 332 (6162) : 360 - 361
[3] PURIFICATION OF THE CHICK EYE CILIARY NEURONOTROPHIC FACTOR
BARBIN, G
MANTHORPE, M
VARON, S
[J]. JOURNAL OF NEUROCHEMISTRY, 1984, 43 (05) : 1468 - 1478
[4] BASIC FIBROBLAST GROWTH-FACTOR IN THE ADRENAL-GLAND - POSSIBLE TROPHIC ROLE FOR PREGANGLIONIC NEURONS INVIVO
BLOTTNER, D
WESTERMANN, R
GROTHE, C
BOHLEN, P
UNSICKER, K
[J]. EUROPEAN JOURNAL OF NEUROSCIENCE, 1989, 1 (05) : 471 - 478
[5] IMMUNOADSORBENT ISOLATION OF TRYPSIN-INHIBITORS FROM CORN AND TEOSINTE SEEDS
CORFMAN, RS
REECK, GR
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1982, 715 (02) : 170 - 174
[6] ENGVALL E, 1972, J IMMUNOL, V109, P129
[7] BRAIN BASIC FIBROBLAST GROWTH-FACTOR STIMULATES THE PROLIFERATION OF RAT NEURONAL PRECURSOR CELLS-INVITRO
GENSBURGER, C
LABOURDETTE, G
SENSENBRENNER, M
[J]. FEBS LETTERS, 1987, 217 (01) : 1 - 5
[8] ISOLATION OF PITUITARY FIBROBLAST GROWTH-FACTOR BY FAST PROTEIN LIQUID-CHROMATOGRAPHY (FPLC) - PARTIAL CHEMICAL AND BIOLOGICAL CHARACTERIZATION
GOSPODAROWICZ, D
MASSOGLIA, S
CHENG, J
LUI, GM
BOHLEN, P
[J]. JOURNAL OF CELLULAR PHYSIOLOGY, 1985, 122 (02) : 323 - 332
[9] MOLECULAR AND BIOLOGICAL CHARACTERIZATION OF FIBROBLAST GROWTH-FACTOR, AN ANGIOGENIC FACTOR WHICH ALSO CONTROLS THE PROLIFERATION AND DIFFERENTIATION OF MESODERM AND NEUROECTODERM DERIVED CELLS
GOSPODAROWICZ, D
NEUFELD, G
SCHWEIGERER, L
[J]. CELL DIFFERENTIATION, 1986, 19 (01): : 1 - 17
[10] BASIC FIBROBLAST GROWTH-FACTOR PROMOTES INVITRO SURVIVAL AND CHOLINERGIC DEVELOPMENT OF RAT SEPTAL NEURONS - COMPARISON WITH THE EFFECTS OF NERVE GROWTH-FACTOR
GROTHE, C
OTTO, D
UNSICKER, K
[J]. NEUROSCIENCE, 1989, 31 (03) : 649 - 661

← 1 2 3 →