VARIABILITY OF HISTONE-H1 IN RABBIT-POPULATIONS

1. 1. Perchloric acid-soluble proteins containing H1 histone as a main component were isolated from whole liver, lung and kidney of New Zealand White, Chinchilla, French Silver and Czech Spotted rabbits. 2. 2. Proteins were resolved in a two-dimensional polyacrylamide slab gel into 4-5 spots depending on the tissue. 3. 3. One of the histone subtypes, Hle, was found to be nonuniformly distributed within rabbit populations. 4. 4. The prevailing fraction of animals had only a single spot of H1e (phenotype A). Approximately 10-28% of animals, depending on the breed, had two spots of H1e (e1 and e2; phenotype B) that slightly differed in the apparent molecular weights. 5. 5. These two distinct gel patterns of H1e showed no tissue specificity, and the same phenotype was revealed in all tissues from the same animal. © 1990.