IRON DETOXIFYING ACTIVITY OF FERRITIN - EFFECTS OF HUMAN H-APOFERRITIN AND L-APOFERRITIN ON LIPID-PEROXIDATION INVITRO

被引：87

作者：

COZZI, A ^{[1
]}

SANTAMBROGIO, P ^{[1
]}

LEVI, S ^{[1
]}

AROSIO, P ^{[1
]}

机构：

[1] UNIV MILANO,SAN RAFFAELE INST,DEPT BIOMED SCI & TECHNOL,VIA OLGETTINA 60,I-20132 MILAN,ITALY

来源：

FEBS LETTERS | 1990年 / 277卷 / 1-2期

关键词：

RECOMBINANT FERRITIN; MUTANT PROTEIN; LIPID PEROXIDATION; IRON TOXICITY;

D O I：

10.1016/0014-5793(90)80823-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three recombinant human apoferritin variants were added to ferrous iron and the amount of lipid peroxidation produced by hydrogen peroxide was studied. The H-apoferritin had the strongest inhibitory effect on lipid peroxidation, probably due to its ferroxidase activity. The L-apoferritin inhibited lipid peroxidation slowly and only at neutral pH. The H-mutant 91, deleted of the last 22 C-terminal amino acids, and which is not able to form an iron core, had minimal effects on iron lipid peroxidation. It was concluded that both ferro-oxidase and iron mineralization activities are necessary for ferritin iron detoxifying action.

引用

页码：119 / 122

页数：4

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