DIFFERENT MECHANISMS OF CA-2+ ENTRY INDUCED BY DEPOLARIZATION AND MUSCARINIC RECEPTOR STIMULATION IN SH-SY5Y HUMAN NEUROBLASTOMA-CELLS

被引：55

作者：

LAMBERT, DG

WHITHAM, EM

BAIRD, JG

NAHORSKI, SR

机构：

[1] Department of Pharmacology and Therapeutics, University of Leicester, Leicester

来源：

MOLECULAR BRAIN RESEARCH | 1990年 / 8卷 / 03期

基金：

英国惠康基金;

关键词：

K[!sup]+[!/sup]-depolarization; Muscarinic receptor stimulation; SH-SY5Y human neuroblastoma cells; Voltage-sensitive Ca[!sup]2+[!/sup] channel; Ca[!sup]2+[!/sup]][!sub]i[!/sub;

D O I：

10.1016/0169-328X(90)90026-A

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Depolarization by elevated K+ and stimulation of muscarinic M3 receptors evoke rises in [Ca2+]i in Fura 2-loaded SH-SY5Y human neuroblastoma cells. The response to K+ (30 and 60 mM) could be inhibited by the dihydropyridine L-channel antagonist +PN 200-110 and totally suppressed by Ni2+, the N-channel blocker ω-conotoxin reduced the response to 60 mM K+. Carbachol-stimulated increase in [Ca2+]i was blocked by atropine and Ni2+ but was totally resistant to the L- and N-channel blockers. This study reveals the presence of L- and N-type voltage-sensitive Ca2+ channels on undifferentiated SH-SY5Y cells that are opened by K+ depolarization but not by muscarinic stimulation. © 1990.

引用

页码：263 / 266

页数：4

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