A UBIQUITYL-CALMODULIN SYNTHETASE THAT EFFECTIVELY RECOGNIZES THE CA-2+-FREE FORM OF CALMODULIN

被引：10

作者：

MAJETSCHAK, M ^{[1
]}

LAUB, M ^{[1
]}

JENNISSEN, HP ^{[1
]}

机构：

[1] UNIV ESSEN GESAMTHSCH,INST PHYSIOL CHEM,HUFELANDSTR 55,W-4300 ESSEN 1,GERMANY

来源：

FEBS LETTERS | 1993年 / 315卷 / 03期

关键词：

CALMODULIN; UBIQUITIN; UBIQUITYL-CALMODULIN SYNTHETASE; PROTEIN UBIQUITINATION; CALCIUM;

D O I：

10.1016/0014-5793(93)81192-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ubiquityl-calmodulin synthetase (uCaM-synthetase) activity as detected in reticulocyte lysate and the crude extracts of rabbit tissues [FEBS Lett. 294 (1991) 229-2331 has been well characterized as being essentially Ca2+-dependent (-Ca2+/+Ca2+ activity ratio: 0.15-0.2). However, during the purification of this enzyme on ubiquitin-Sepharose the Ca2+-dependent activity is lost and an essentially Ca2+-independent enzyme (-Ca2+/+Ca2+ activity ratio: 1.0-1.5) is obtained which was purified 90-fold (uCaM-Syn F1) to a final specific activity of 0.32 pkat/mg. During the purification procedure a second protein factor (uCaM-Syn F2) was isolated that has no catalytic activity by itself but restores Ca2+ dependence to the uCaM-Syn F1 fraction (-Ca2+/+Ca2+ activity ratio: 0.1) and enhances the catalytic activity in uCaM-Syn F1 in the presence of Ca2+ over 40-fold. It is concluded that several (possibly interdependent) forms of uCaM-synthetase exist which display different substrate specificities for calmodulin.

引用

页码：347 / 352

页数：6

共 21 条

[1] LARGE-SCALE PURIFICATION AND CHARACTERIZATION OF CALMODULIN FROM RAM TESTIS - ITS METAL-ION-DEPENDENT CONFORMERS [J].

AUTRIC, F ;

FERRAZ, C ;

KILHOFFER, MC ;

CAVADORE, JC ;

DEMAILLE, JG .

BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 631 (01) :139-147

[2] ATP-DEPENDENT CONJUGATION OF RETICULOCYTE PROTEINS WITH THE POLYPEPTIDE REQUIRED FOR PROTEIN-DEGRADATION [J].

CIECHANOVER, A ;

HELLER, H ;

ELIAS, S ;

HAAS, AL ;

HERSHKO, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (03) :1365-1368

[3] HEAT-STABLE POLYPEPTIDE COMPONENT OF AN ATP-DEPENDENT PROTEOLYTIC SYSTEM FROM RETICULOCYTES [J].

CIECHANOVER, A ;

HOD, Y ;

HERSHKO, A .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1978, 81 (04) :1100-1105

[4] ATP-DEPENDENT PROTEOLYSIS AND THE ROLE OF UBIQUITIN IN RABBIT CARDIAC-MUSCLE [J].

GEHRKE, PP ;

JENNISSEN, HP .

BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1987, 368 (06) :691-708

[5]

GEHRKE PP, 1987, BIOL CHEM H-S, V368, P744

[6] ON THE ANTIQUITY OF INTRONS [J].

GILBERT, W ;

MARCHIONNI, M ;

MCKNIGHT, G .

CELL, 1986, 46 (02) :151-154

[7]

HERSHKO A, 1986, J BIOL CHEM, V261, P1992

[8] UBIQUITIN-CALMODULIN CONJUGATING ACTIVITY FROM CARDIAC-MUSCLE [J].

JENNISSEN, HP ;

LAUB, M .

BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1988, 369 (12) :1325-1330

[9] CA2+-DEPENDENT UBIQUITINATION OF CALMODULIN IN YEAST [J].

JENNISSEN, HP ;

BOTZET, G ;

MAJETSCHAK, M ;

LAUB, M ;

ZIEGENHAGEN, R ;

DEMIROGLOU, A .

FEBS LETTERS, 1992, 296 (01) :51-56

[10] A NOVEL, ARSENITE-SENSITIVE E2 OF THE UBIQUITIN PATHWAY - PURIFICATION AND PROPERTIES [J].

KLEMPERER, NS ;

BERLETH, ES ;

PICKART, CM .

BIOCHEMISTRY, 1989, 28 (14) :6035-6041

← 1 2 3 →