ADENOSINE RECEPTOR-MEDIATED MODULATION OF ACETYLCHOLINE-RELEASE FROM RAT STRIATAL SYNAPTOSOMES

1 The effects of A1 and A2a adenosine receptor agonists on the veratridine-evoked release of [H-3]-acetylcholine [H-3]-ACh) from rat striatal synaptosomes was investigated by use of the A1-selective agonist, R-PIA and the 185 fold selective A2a agonist, CGS 21680. The effects of NECA, which is equipotent at both receptor subtypes, were also studied. 2 The evoked release of [H-3]-ACh was significantly enhanced by the A2a agonist CGS 21680 but decreased by the A1 agonist, R-PIA. The effects of NECA were dependent on the concentration used, with high concentrations inhibiting and low concentrations enhancing the evoked release of [H-3]-ACh. In the absence of any antagonists, the rank order of potency for these three drugs on increasing [H-3]-ACh release was CGS 21680>NECA>R-PIA. 3 The stimulatory effects of CGS 21680 and low NECA concentrations on evoked [H-3]-ACh release were antagonized by the A2a receptor antagonists, CP66,713 (300 nm) and CGS 15943A (50 nM) whilst the inhibitory effects of R-PIA were reversed by the selective A1 antagonist, DPCPX (4 nM). In the presence of DPCPX, NECA greatly enhanced the evoked release of [H-3]-ACh at all concentrations studied when, during such A1 receptor blockade, the rank order of potency was NECA >> CGS 21680 > R-PIA. 4 These results demonstrate that both A1 and A2a adenosine receptors modulate the veratridine-evoked release of [H-3]-ACh from rat striatal synaptosomes.