ADENOVIRUS SEROTYPE-3 FIBER PROTEIN IS EXPRESSED AS A TRIMER IN ESCHERICHIA-COLI

被引：9

作者：

ALBIGES-RIZO, C ^{[1
]}

CHROBOCZEK, J ^{[1
]}

机构：

[1] INST MAX VON LAUE PAUL LANGEVIN, CNRS, URA 1333, F-38042 GRENOBLE, FRANCE

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1990年 / 212卷 / 02期

关键词：

D O I：

10.1016/0022-2836(90)90121-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The adenovirus serotype 3 (Ad3) fibre has been expressed in Escherichia coli as an insoluble protein. The protein was solubilized by extraction with urea. Slow removal of urea during the purification procedure resulted in a soluble Ad3 fibre preparation. Polyacrylamide gel analysis of the purified fibre protein, as well as cross-linking experiments performed on cellular debris of expressing cells, suggest that the recombinant Ad3 fibre self-assembles as a trimer from identical polypeptide chains. Gel filtration gave the same exclusion volume for the purified recombinant fibre and for the native fibre in the protein mixture extracted from the Ad3-infected cells. The recombinant fibre was partially resistant to proteolytic degradation, suggesting a folded structure. © 1990.

引用

页码：247 / 252

页数：6

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