SYNERGISTIC EFFECT OF HUMAN LYSOZYME PLUS AMPICILLIN OR BETA-LYSIN ON THE KILLING OF LISTERIA-MONOCYTOGENES

被引:12
作者
ASENSI, V
FIERER, J
机构
[1] VET ADM MED CTR,DEPT MED,INFECT DIS SECT 111F,3350 LA JOLLA VILLAGE DR,SAN DIEGO,CA 92161
[2] UNIV CALIF SAN DIEGO,SCH MED,LA JOLLA,CA 92093
[3] VET ADM MED CTR,DEPT PATHOL,SAN DIEGO,CA 92161
关键词
D O I
10.1093/infdis/163.3.574
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Although ampicillin is often only bacteriostatic for Listeria monocytogenes in vitro, serum from ampicillin-treated patients was bactericidal. The bactericidal effect of serum was partly removed by bentonite, Seitz-filtration, and addition of FeCl3, suggesting it is mediated by lysozyme and beta-lysin. Partly purified human beta-lysin plus purified human lysozyme or either protein plus ampicillin were bactericidal for L. monocytogenes. Hen egg white lysozyme was not active. Lysozyme and beta-lysin were not synergistic with tetracycline, trimethoprim/sulfamethoxazole, or chloramphenicol. Thus, lysozyme and beta-lysin may play a role in the natural resistance to L. monocytogenes, and these serum proteins could contribute to the effectiveness of ampicillin in vivo.
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页码:574 / 578
页数:5
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