EFFECTS OF A VARIETY OF CYTOKINES AND INDUCING AGENTS ON VASCULAR-PERMEABILITY FACTOR MESSENGER-RNA LEVELS IN U937 CELLS

被引：65

作者：

DOLECKI, GJ

CONNOLLY, DT

机构：

[1] Health Sciences Division, Monsanto Corporate Research, Monsanto Company, St. Louis

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 180卷 / 02期

关键词：

D O I：

10.1016/S0006-291X(05)81103-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vascular permeability factor (VPF) is an approximately 40-kDa disulfide-linked dimeric glycoprotein that is active in increasing blood vessel permeability, endothelial cell growth and angiogenesis. Little is known about VPF gene regulation. In this study, we investigated the effects of a variety of cytokines and inducing agents on VPF mRNA levels in the monocyte-like U937 cell line. Transforming growth factor-beta 1 caused a 1.8-fold increase in VPF mRNA levels after 4 hours, followed by a decline to basal levels by 18 hours. Phorbol 12-myristate 13-acetate, a potent inducer of the differentiation of U937 cells, caused a 12.5-fold increase in VPF mRNA levels at 24 hours, coinciding with the differentiation of these monocyte-like cells into macrophage-like cells. © 1991 Academic Press, Inc.

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页码：572 / 578

页数：7

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