ENZYMATIC PATHWAYS INVOLVED IN CELL RESPONSE TO H2O2

被引：6

作者：

LINK, EM

机构：

[1] Department of Chemical Pathology, Academic Unit, University College and Middlesex School of Medicine, London W1P 6DB, Cleveland Street

来源：

FREE RADICAL RESEARCH COMMUNICATIONS | 1990年 / 11卷 / 1-3期

关键词：

Cyclooxygenase; Enzymic pathway interaction; Glutathione peroxidase; Hydrogen peroxide cytotoxicity; Indomethacin;

D O I：

10.3109/10715769009109671

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An influence of possible interaction of glutathione peroxidase and cyclooxygenase on the clonogenic survival of epithelial cells exposed in vitro to H2O2 was investigated. Indomethacin served as the inhibitor of cyclooxygenase, and the use of alkaline (7.5) or acidic (6.5) pH combined with controlled supply of glucose modified glutathione peroxidase activity. Indomethacin affected survival of cells exposed to H2O2 in a biphasic manner, enhancing cytotoxicity at lower hydrogen peroxide concentrations, and diminishing it at higher concentrations. The turning point moved gradually to higher concentrations of H2O2 corresponding to the augmented decomposition of hydrogen peroxide caused by increased activity of glutathione peroxidase. The data revealed that both enzymic pathways interact in the presence of H2O2, resulting in the overall cell survival different from that obtained after inhibition of either. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：89 / 97

页数：9

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