POLYAMINE-PYRIDOXAL SCHIFF-BASES IN URINE

被引:20
作者
AIGNERHELD, R [1 ]
CAMPBELL, RA [1 ]
DAVES, GD [1 ]
机构
[1] UNIV OREGON,HLTH SCI CTR,PEDIAT RENAL METAB LAB,PORTLAND,OR 97201
关键词
D O I
10.1073/pnas.76.12.6652
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Schiff bases of the diamines 1,3-diaminopropane, putrescine, and cadaverine and the polyamines spermidine and spermine with pyridoxal or pyridoxal phosphate occur in human urine, as shown by gas chromatographic/mass spectrometric selected ion-monitoring techniques. By use of synthetic standards, procedures were devised for conversion of the Schiff bases to stable derivatives amenable to gas chromatographic/mass spectrometric analysis. These procedures involve borohydride reduction of the C=N double bond, hydrolytic removal of the phosphate group, chromatographic separation from the bulk of urinary constituents, and trifluoroacetylation of polar functional groups. The levels of the polyamine-pyridoxal Schiff bases were estimated to be in the range of pmol/ml of urine.
引用
收藏
页码:6652 / 6655
页数:4
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