TRIGLYCERIDE HYDROLYSIS AND STABILITY OF A RECOMBINANT CUTINASE FROM FUSARIUM-SOLANI IN AOT-ISO-OCTANE REVERSED MICELLES

被引：45

作者：

MELO, EP ^{[1
]}

AIRESBARROS, MR ^{[1
]}

CABRAL, JMS ^{[1
]}

机构：

[1] Univ Tecn Lisboa, ENGN BIOQUIM LAB, INST SUPER TECN, P-1000 LISBON, PORTUGAL

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1995年 / 50卷 / 01期

关键词：

RECOMBINANT CUTINASE; REVERSED MICELLES; TRIGLYCERIDE HYDROLYSIS; STABILITY;

D O I：

10.1007/BF02788039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A recombinant cutinase from Fusarium solani was encapsulated in AOT reversed micelles. Physicochemical parameters of the system were optimized relative to triolein hydrolysis. Kinetic studies of triglyceride hydrolysis showed a decrease in specificity with increase of the acyl chain length. Stability of cutinase in the system under study is lower than in aqueous solution and decreases with increase in the water content in the system (W-0 = [H2O]/[AOT]). The products of triolein hydrolysis had little effect on the cutinase stability. Although glycerol did not alter the stability, oleic acid decreases the enzyme stability. The increase in log P of solvent (from iso-octane to n-dodecane) decreased the stability. Deactivation profiles were fitted with the Henley and Sadana model (1).

引用

页码：45 / 56

页数：12

共 23 条

[1] FORMATION OF WATER-OIL-MICROEMULSIONS [J].

EICKE, HF ;

REHAK, J .

HELVETICA CHIMICA ACTA, 1976, 59 (08) :2883-2891

[2] ACTIVITY OF LIPASE IN WATER-IN-OIL MICROEMULSIONS [J].

FLETCHER, PDI ;

ROBINSON, BH ;

FREEDMAN, RB ;

OLDFIELD, C .

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS I, 1985, 81 :2667-2679

[3] CHARACTERISTICS OF LIPASE-CATALYZED HYDROLYSIS OF OLIVE OIL IN AOT-ISOOCTANE REVERSED MICELLES [J].

HAN, D ;

RHEE, JS .

BIOTECHNOLOGY AND BIOENGINEERING, 1986, 28 (08) :1250-1255

[4] ESTERIFICATION REACTIONS OF LIPASE IN REVERSE MICELLES [J].

HAYES, DG ;

GULARI, E .

BIOTECHNOLOGY AND BIOENGINEERING, 1990, 35 (08) :793-801

[5] 1-MONOGLYCERIDE PRODUCTION FROM LIPASE-CATALYZED ESTERIFICATION OF GLYCEROL AND FATTY-ACID IN REVERSE MICELLES [J].

HAYES, DG ;

GULARI, E .

BIOTECHNOLOGY AND BIOENGINEERING, 1991, 38 (05) :507-517

[6] CATEGORIZATION OF ENZYME DEACTIVATIONS USING A SERIES-TYPE MECHANISM [J].

HENLEY, JP ;

SADANA, A .

ENZYME AND MICROBIAL TECHNOLOGY, 1985, 7 (02) :50-60

[7]

Kolattukudy P. E., 1984, LIPASES, P471, DOI [10.1016/0076-6879(81)71078-4, DOI 10.1016/0076-6879(81)71078-4]

[8]

LAANE C, 1987, METHOD ENZYMOL, V136, P216

[9]

Laane C., 1987, BIOCATALYSIS ORGANIC, P65

[10]

LAUWEREYS M, 1991, LIPASES STRUCTURE ME, V16, P243

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