DETECTION OF THE SCHISTOSOME CIRCULATING CATHODIC ANTIGEN BY ENZYME-IMMUNOASSAY USING BIOTINYLATED MONOCLONAL-ANTIBODIES

被引：88

作者：

DEJONGE, N ^{[1
]}

KREMSNER, PG ^{[1
]}

KRIJGER, FW ^{[1
]}

SCHOMMER, G ^{[1
]}

FILLIE, YE ^{[1
]}

KORNELIS, D ^{[1
]}

VANZEYL, RJM ^{[1
]}

VANDAM, GJ ^{[1
]}

FELDMEIER, H ^{[1
]}

DEELDER, AM ^{[1
]}

机构：

[1] LANDESINST TROP MED BERLIN, W-1000 BERLIN 19, GERMANY

来源：

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 1990年 / 84卷 / 06期

关键词：

D O I：

10.1016/0035-9203(90)90094-U

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

We have developed an enzyme immunoassay (ELISA) for the quantification of the schistosome circulating cathodic antigen (CCA), a glycoprotein associated with the syncitium lining the gut of the parasite. A mouse monoclonal antibody of IgG3 isotype was used as coating (antigen-capture) antibody, while a biotinylated mouse monoclonal IgM was used as second (antigen-detecting) antibody. Streptavidin-alkaline phosphatase was used as enzyme label. The lower detection limit of the assay was 1.0 ng of the trichloroacetic acid soluble fraction of adult worm antigen (AWA-TCA) per ml, which corresponds to approximately 0.2 ng CCA per ml. The ELISA showed a linear range from 1.0 to 62.5 ng AWA-TCA per ml. Serum and urine samples of 16 individuals infected with Schistosoma mansoni (egg counts ranging from 5 to 4820 eggs per gram of faeces) were tested in the assay. Antigen titres ranged from < 4-8192. This assay represents a considerable advantage in diagnosis of Schistosoma infections as it allows the detection and quantification of CCA in serum and urine in even lightly infected individuals.

引用

页码：815 / 818

页数：4

共 32 条

[1] SCHISTOSOMA-MANSONI - DETECTION AND CHARACTERIZATION OF ANTIGENS AND ANTIGENEMIA BY INHIBITION ENZYME-LINKED IMMUNOSORBENT-ASSAY (IELISA) [J].