A rapid and sensitive determination of paclitaxel in rat plasma by UPLC-MS/MS method: Application to a pharmacokinetic study

被引：14

作者：

Lian, He ^{[1
]}

Sun, Jin ^{[1
]}

Zhang, Tianhong ^{[1
]}

机构：

[1] Shenyang Pharmaceut Univ, Sch Pharm, Dept Biopharmaceut, Mailbox 59,103,Wenhua Rd, Shenyang 110016, Liaoning, Peoples R China

来源：

ASIAN JOURNAL OF PHARMACEUTICAL SCIENCES | 2013年 / 8卷 / 03期

关键词：

Paclitaxel; UPLC-MS/MS method; Liquid-liquid extraction; Pharmacokinetic study;

D O I：

10.1016/j.ajps.2013.07.026

中图分类号：

R9 [药学];

学科分类号：

1007 [药学];

摘要：

A rapid and sensitive method for quantitative determination of paclitaxel in rat plasma was developed and validated by using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Docetaxel was used as an internal standard and diethyl ether was the liquid-liquid extraction agent. Multiple reaction monitoring (MRM) mode via positive electrospray ionization (ESI) was applied to detect paclitaxel and IS at the transitions m/z 854 -> 286 and m/z 808.48 -> 527.3, respectively. This method covered a linearity range from 5 to 5000 ng/ml, with the total run time of 3.0 min. In summary, a high-throughout UPLC-MS/MS method was successfully developed to measure paclitaxel in rat plasma and was applied to pharmacokinetic study after intravenous administration of paclitaxel. (C) 2013 Shenyang Pharmaceutical University. Production and hosting by Elsevier B.V. All rights reserved.

引用

页码：199 / 205

页数：7

共 14 条

[1]

Arbuck S G, 1993, J Natl Cancer Inst Monogr, P11

[2]

Randomised phase 3 open-label trial of first-line treatment with gemcitabine in association with docetaxel or paclitaxel in women with metastatic breast cancer: a comparison of different schedules and treatments [J].