ISOLATION AND CHARACTERIZATION OF A NEW FACULTATIVELY METHYLOTROPHIC BACTERIUM - DESCRIPTION OF METHYLORHABDUS-MULTIVORANS, GEN-NOV, SP-NOV

被引：46

作者：

DORONINA, NV

BRAUSSTROMEYER, SA

LEISINGER, T

TROTSENKO, YA

机构：

[1] RUSSIAN ACAD SCI,INST BIOCHEM & PHYSIOL MICROORGANISMS,PUSHCHINO 142292,RUSSIA

[2] ETH ZENTRUM,INST MIKROBIOL,CH-8092 ZURICH,SWITZERLAND

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 1995年 / 18卷 / 01期

关键词：

METHYLORHABDUS MULTIVORANS; FACULTATIVE METHYLOTROPH; DICHLOROMETHANE; SERINE PATHWAY;

D O I：

10.1016/S0723-2020(11)80454-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new Gram-negative aerobic, asporogenous, nonmotile, colourless, facultatively methylotrophic bacterium with the serine pathway of C1 assimilation is described. The organism (strain DM13) is a short rod multiplying by fission with the formation of a constriction connecting freshly divided cells. The G+C content of DNA is 66,2 mol %. The major cellular fatty acids are cis-vaccenic, lactobacillic and palmitic acid. The major ubiquinone is Q-10. The bacterium utilizes dichloromethane, methanol and methylamine as C1 sources of carbon and energy as well as a wide range of polycarbon compounds. It shows a low degree of DNA-DNA similarity (<10%) with representatives of the genera Pseudomonas, Methylobacterium, Methylomicrobium, Hyphomicrobium, and Aminobacter. Based on morphological, pheno- and genotypic distinctions, the isolate is proposed to represent a new genus - Methylorhabdus with the type strain Methylorhabdus multivorans gen. nov., sp. nov. The characteristics of the new genus and species are presented.

引用

页码：92 / 98

页数：7

共 17 条

[1]

Bader R., Leisinger T., Isolation and characterization of the Methylophilus sp. DM11 gene encoding dichloromethane dehalogenase/glutathione S-transferase, J. Bacteriol., 176, pp. 3466-3473, (1994)

[2]

Krieg N.R., Bergey's Manual of Systematic Bacteriology, (1989)

[3]

Bousfield I.J., Green P.N., Reclassification of bacteria of the genus Protomonas Urakami and Komagara 1984 in the genus Methylobacterium (Part, Cole, and Hanson) emend. Green and Bousfield 1983, International Journal of Systematic Bacteriology, 35, (1985)

[4]

Collins M.D., Analysis of isoprenoid quinones, Methods in Microbiology, pp. 329-366, (1985)

[5]

Doronina N.V., Trotsenko Y.A., Mikrobiologiya, (1994)

[6]

Doronina N.V., Govorukhina N.I., Lysenko A.M., Trotsenko Y.A., Analysis of DNA-DNA homology in obligate methylotrophic bacteria, Mikrobiologiya (English translation), 57, pp. 509-517, (1987)

[7]

Doronina N.V., Trotsenko Y.A., Leisinger T., Metabolic characterization of aerobic dichloromethane utilizers, Abstract A13, 7th Internat. Symp. on Microbial Growth on C1 Compounds, Warwick, UK, (1992)

[8]

Doronina N.V., Govorukbina N.I., Trotsenko Y.A., Enzymes of ammonia assimilation in bacteria with various pathways of C1 metabolism, Mikrobiologiya (English translation), 51, pp. 31-35, (1983)

[9]

Galli R., Leisinger T., Plasmid analysis and cloning of the dichloromethane-utilization genes of Methylobacterium sp. DM4, J. Gen. Microbiol., 134, pp. 943-952, (1988)

[10]

Govorukhina N.I., Doronina N.V., Andreyev L.V., Trotsenko Y.A., Methylomicrobium: a new genus of facultatively methylotrophic bacteria, Mikrobiologiya (English translation), 58, pp. 260-266, (1989)

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