THE SIMULIUM-DAMNOSUM SPECIES COMPLEX - PHYLOGENETIC ANALYSIS AND MOLECULAR-IDENTIFICATION BASED UPON MITOCHONDRIALLY ENCODED GENE-SEQUENCES

被引：50

作者：

TANG, J

TOE, L

BACK, C

ZIMMERMAN, PA

PRUESS, K

UNNASCH, TR

机构：

[1] UNIV ALABAMA, DIV GEOG MED, BIRMINGHAM, AL 35294 USA

[2] NIH, PARASIT DIS LAB, BETHESDA, MD 20892 USA

[3] UNIV NEBRASKA, DEPT ENTOMOL, LINCOLN, NE 68583 USA

[4] ONCHOCERCIASIS CONTROL PROGRAM, BOUAKE, COTE IVOIRE

来源：

INSECT MOLECULAR BIOLOGY | 1995年 / 4卷 / 02期

关键词：

SIMULIUM DAMNOSUM SL; DIRECTED HETERODUPLEX ANALYSIS; MITOCHONDRIAL DNA; PHYLOGENY;

D O I：

10.1111/j.1365-2583.1995.tb00011.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The DNA sequence of portions of the 16s rRNA and the NADH dehydrogenase subunit 4 (ND4) genes were used to determine phylogenetic relationships in the Simulium damnosum s.l. species complex. Results suggested that at least two major clades existed in the S. damnosum species complex, and that members of the S. damnosum s.l, species complex were not closely related to North American Simulium species. The sequence variability of the ND4 gene was exploited to develop a method to distinguish the sibling species of the S. damnosum s.l. species complex, based on directed heteroduplex analysis of PCR products derived from the ND4 gene. This method was capable of classifying the six sibling species into at least five groups.

引用

页码：79 / 88

页数：10

共 30 条

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