A BASE-PAIR BETWEEN TRANSFER-RNA AND 23S RIBOSOMAL-RNA IN THE PEPTIDYL TRANSFERASE CENTER OF THE RIBOSOME

被引：219

作者：

SAMAHA, RR ^{[1
]}

GREEN, R ^{[1
]}

NOLLER, HF ^{[1
]}

机构：

[1] UNIV CALIF SANTA CRUZ, SINSHEIMER LABS, CTR MOLEC BIOL RNA, SANTA CRUZ, CA 95064 USA

来源：

NATURE | 1995年 / 377卷 / 6547期

关键词：

D O I：

10.1038/377309a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Interaction of the conserved CCA terminus of tRNA with rRNA in the peptidyl transferase P site has been studied by in vitro genetics. A Watson-Crick G-C pair between G2252 in a conserved hairpin loop of 235 rRNA and C74 at the acceptor end of tRNA is required for proper functional interaction of the CCA end of tRNA with the ribosomal P site. These findings establish a direct role for 23S rRNA in protein synthesis.

引用

页码：309 / 314

页数：6

共 39 条

[1] SPECIFIC STRUCTURAL PROBING OF PLASMID-CODED RIBOSOMAL-RNAS FROM ESCHERICHIA-COLI [J].

AAGAARD, C ;

ROSENDAHL, G ;

DAM, M ;

POWERS, T ;

DOUTHWAITE, S .

BIOCHIMIE, 1991, 73 (12) :1439-1444

[2] IDENTIFICATION OF A SITE ON 23S RIBOSOMAL-RNA LOCATED AT THE PEPTIDYL TRANSFERASE CENTER [J].

BARTA, A ;

STEINER, G ;

BROSIUS, J ;

NOLLER, HF ;

KUECHLER, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (12) :3607-3611

[3] AFFINITY LABELING OF SPECIFIC REGIONS OF 23 S RNA BY REACTION OF "N-BROMOACETYL-PHENYLALANYL-TRANSFER RNA WITH ESCHERICHIA-COLI RIBOSOMES [J].

BREITMEYER, JB ;

NOLLER, HF .

JOURNAL OF MOLECULAR BIOLOGY, 1976, 101 (03) :297-306

[4] BINDING OF S-RNA BY ESCHERICHIA COLI RIBOSOMES [J].

CANNON, M ;

KRUG, R ;

GILBERT, W .

JOURNAL OF MOLECULAR BIOLOGY, 1963, 7 (04) :360-&

[5] THE DECODING REGION OF 16S RNA - A CROSS-LINKING STUDY OF THE RIBOSOMAL A-SITE, P-SITE AND E-SITE USING TRANSFER-RNA DERIVATIZED AT POSITION-32 IN THE ANTICODON LOOP [J].

DORING, T ;

MITCHELL, P ;

OSSWALD, M ;

BOCHKARIOV, D ;

BRIMACOMBE, R .

EMBO JOURNAL, 1994, 13 (11) :2677-2685

[6] COMPARISON OF ESCHERICHIA-COLI TRANSFER RNAPHE IN THE FREE STATE, IN THE TERNARY COMPLEX AND IN THE RIBOSOMAL-A AND RIBOSOMAL-P SITES BY CHEMICAL PROBING [J].

DOUTHWAITE, S ;

GARRETT, RA ;

WAGNER, R .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1983, 131 (02) :261-269

[7]

Evnin L B, 1988, Ann N Y Acad Sci, V542, P61, DOI 10.1111/j.1749-6632.1988.tb25808.x

[8] INTERACTION OF TRANSFER-RNA WITH 30-S SUBUNITS OF RIBOSOMES IN ABSENCE OF MESSENGER [J].

GRAJEVSKAJA, RA ;

ODINZOV, VB ;

SAMINSKY, EM ;

BRESLER, SE .

FEBS LETTERS, 1973, 33 (01) :11-14

[9] MUTATIONS IN THE PEPTIDYL TRANSFERASE REGION OF ESCHERICHIA-COLI 23S RIBOSOMAL-RNA AFFECTING TRANSLATIONAL ACCURACY [J].

GREGORY, ST ;

LIEBERMAN, KR ;

DAHLBERG, AE .

NUCLEIC ACIDS RESEARCH, 1994, 22 (03) :279-284

[10] PREPARATIVE INVITRO MESSENGER-RNA SYNTHESIS USING SP6 AND T7 RNA-POLYMERASES [J].

GUREVICH, VV ;

POKROVSKAYA, ID ;

OBUKHOVA, TA ;

ZOZULYA, SA .

ANALYTICAL BIOCHEMISTRY, 1991, 195 (02) :207-213

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