ANALYSIS OF RADIOACTIVE AND NON-RADIOACTIVE PURINE-BASES, NUCLEOSIDES, AND NUCLEOTIDES BY HIGH-SPEED CHROMATOGRAPHY ON A SINGLE COLUMN

被引：45

作者：

BAKAY, B

NISSINEN, E

SWEETMAN, L

机构：

[1] Department of Pediatrics, University of California at San Diego, La Jolla

来源：

ANALYTICAL BIOCHEMISTRY | 1978年 / 86卷 / 01期

关键词：

D O I：

10.1016/0003-2697(78)90319-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The concentrations of radioactive and nonradioactive purine bases, purine nucleosides, purine mono-, di-, and trinucleotides in acid extracts of fibroblasts were determined by anion-exchange column chromatography. The concentrations of nonradioactive components were determined by computerized integration of the signal from a double-beam uv-detector. The radioactive metabolites were quantitated by high-efficiency, continuous liquid scintillation counting, employing a discrete sample transport system. © 1978.

引用

页码：65 / 77

页数：13

共 15 条

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