ROTATIONAL RESOLUTION OF METHYL-GROUP SUBSTITUTION AND ANISOTROPIC ROTATION OF FLAVINS AS REVEALED BY PICOSECOND-RESOLVED FLUORESCENCE DEPOLARIZATION

被引：7

作者：

LEENDERS, R

BASTIAENS, P

LUNSCHE, R

VANHOEK, A

VISSER, AJWG

机构：

[1] Department of Biochemistry, Agricultural University, 6703 HA Wageningen

来源：

CHEMICAL PHYSICS LETTERS | 1990年 / 165卷 / 04期

关键词：

D O I：

10.1016/0009-2614(90)87195-W

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The rotational correlation times of the natural flavin compounds riboflavin and FMN and the flavin models lumiflavin, tetra-O-acetyl-riboflavin and their N (3)-methylated derivatives, dissolved in water, were determined with the time-resolved fluorescence depolarization technique. Owing to the time resolution the effect of methyl group substitution could be determined as a few picoseconds longer correlation time, in agreement with the slightly larger molecular volume. Anisotropic rotation of lumiflavin was demonstrated by observing the change in correlation time when excitation was in the second excited singlet state of the flavin. The angle between the emission transition moment and the long axis of the flavin ring system is approximately 22°. © 1990.

引用

页码：315 / 322

页数：8

共 25 条

[1]

BASTIAENS P, IN PRESS

[2] TIME RESPONSE OF VARIOUS TYPES OF PHOTOMULTIPLIERS AND ITS WAVELENGTH DEPENDENCE IN TIME-CORRELATED SINGLE-PHOTON COUNTING WITH AN ULTIMATE RESOLUTION OF 47-PS FWHM [J].