AMINO-ACID-SEQUENCE OF A 12-KDA INHIBITOR OF PROTEIN-KINASE-C

被引：25

作者：

MOZIER, NM

ZURCHERNEELY, HA

GUIDO, DM

MATHEWS, WR

HEINRIKSON, RL

FRASER, ED

WALSH, MP

PEARSON, JD

机构：

[1] UPJOHN CO,DEPT BIOCHEM,7240-209-624,KALAMAZOO,MI 49001

[2] UNIV CALGARY,DEPT MED BIOCHEM,CALGARY T2N 1N4,ALBERTA,CANADA

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1990年 / 194卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1990.tb19421.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complete primary structure of a bovine‐brain‐derived inhibitor of protein kinase C has been established. Fragments of the purified protein were obtained by cleavage with cyanogen bromide, Staphylococcus aureus V8 protease, trypsin and chymotrypsin. Subsequent analysis of the resulting fragments by fast‐atom‐bombardment mass spectrometry and Edman degradation revealed a calculated molecular mass of 11779 Da with the following 107‐amino‐acid sequence: (Formula Presented.) This inhibitor does not share significant primary structural identity with any other known protein. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：19 / 23

页数：5

共 20 条

[1] PREDICTION OF PROTEIN CONFORMATION [J].