USE OF A REPLICA-PLATE ASSAY FOR THE RAPID ASSESSMENT OF SALIVARY PROTEIN-BACTERIA INTERACTIONS

被引：16

作者：

TSENG, CC

SCANNAPIECO, FA

LEVINE, MJ

机构：

[1] Department of Oral Biology, Dental Research Institute, School of Dental Medicine, State University of New York at Buffalo

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 1992年 / 7卷 / 01期

关键词：

SALIVA; AMYLASE; PROLINE-RICH GLYCOPROTEIN; DENTAL PLAQUE; STREPTOCOCCUS-GORDONII; ACTINOMYCES-VISCOSUS;

D O I：

10.1111/j.1399-302X.1992.tb00021.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

A replica-plate assay was used to screen for the interaction of salivary molecules with dental plaque bacteria. Bacterial colonies cultured from supragingival plaque on sheep-blood (SB) agar were replica-plated onto nitrocellulose membranes overlaying SB or mitis-salivarius agar. Membranes with attached colonies were removed and incubated with I-125-amylase or I-125-proline-rich glycoprotein (PRG). Positive interactions were detected by autoradiography. Only strains of Streptococcus gordonii and Actinomyces viscosus bound amylase. and strains of A. viscosus bound PRG. The results suggest that amylase and PRG bind to selected species of aerobic dental plaque bacteria.

引用

页码：53 / 56

页数：4

共 32 条

[1]

Bergey EJ, Levine MJ, Reddy MS, Bradway SD, Al-Hashimi I., Use of the photoaffinity cross‐linking agent N‐hydroxysuccinimidyl‐4‐azidosalicylic acid to characterize salivary glycoprotein bacterial interactions, Biochem J, 234, pp. 43-48, (1984)

[2]

Bolton A, Hunter WM, The labelling of proteins to high specific radioactivities by conjugation to a <sup>125</sup>I‐containing acylating agent, Biochem J, 133, pp. 529-538, (1973)

[3]

Brack CM, Reynolds EC, Characterization of a rat salivary sialoglycoprotein complex which agglutinates Streptococcus mutans, Infect Immun, 55, pp. 1264-1273, (1987)

[4]

Brown MRW, Williams P., The influence of the environment on envelope properties affecting survival of bacteria in infections, Ann Rev Microbiol, 39, pp. 527-556, (1985)

[5]

Campbell LK, Knox KW, Wicken AJ, Influence of growth conditions on adherence of Streptococcus mutans Ingbritt to saliva‐coated hydroxyapatite, Infect Immun, 39, pp. 445-448, (1983)

[6]

Clark WB, Beem JE, Nesbitt WE, Cisar JO, Tseng CC, Levine MJ, Pellicle receptors for Actinomyces viscosus type 1 fimbriae in vitro, Infect Immun, 57, pp. 3003-3008, (1989)

[7]

Coykendall A., Classification and identification of the viridans streptococci, Clin Microbiol Rev, 2, pp. 315-328, (1989)

[8]

Curtis FP, Renn DW, The RepliPlate® colony transfer pad: a new device for replicating microbial colonies, Biotechniques, pp. 152-155, (1985)

[9]

Douglas CWI, The binding of human salivary α‐amylase by oral strains of streptococcal bacteria, Arch Oral Biol, 28, pp. 567-573, (1983)

[10]

Douglas CWI, Characterization of the α‐amylase receptor of Streptococcus gordonii NCTC 7868, J Dent Res, 69, pp. 1746-1752, (1990)

← 1 2 3 4 →