RHIZOSPHERE BACTERIA ANTAGONISTIC TO SOYBEAN CYST (HETERODERA-GLYCINES) AND ROOT-KNOT (MELOIDOGYNE-INCOGNITA) NEMATODES - IDENTIFICATION BY FATTY-ACID ANALYSIS AND FREQUENCY OF BIOLOGICAL-CONTROL ACTIVITY

被引:80
作者
KLOEPPER, JW [1 ]
RODRIGUEZKABANA, R [1 ]
MCINROY, JA [1 ]
YOUNG, RW [1 ]
机构
[1] AUBURN UNIV,ALABAMA AGR EXPT STN,AUBURN,AL 36849
关键词
ANTAGONISTIC PLANTS; BIOLOGICAL CONTROL; FATTY ACIDS; HETERODERA-GLYCINES; MELOIDOGYNE-INCOGNITA; RHIZOBACTERIA; RHIZOSPHERE; SOYBEAN;
D O I
10.1007/BF00012844
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Rhizosphere bacteria were isolated from roots of young and mature plants with known antagonism to phytopathogenic nematodes, including velvet bean (Mucuna deeringiana), castor bean (Ricinus communis), sword bean (Cannavalia ensiformis), and Abruzzi rye (Secale cereale). Isolates from antagonistic plants were compared to soybean isolates for the frequency of antagonism to the root-knot (Meloidogyne incognita) and soybean cyst (Heterodera schachtii) nematodes in a disease assay with soybean. Bacterial isolates were identified using fatty acid analysis, and isolates which exhibited a significant reduction in incidence of soybean damage from both nematodes were characterized physiologically. The bacterial taxa associated with antagonistic plants were markedly different from soybean bacteria. Isolates from soybean were predominantly Bacillus spp., while those from antagonistic plants included more coryneform and Gram-negative genera. Pseudomonas cepacia and Pseudomonas gladioli were predominant among Gram-negative bacteria on antagonistic plants but were not isolated from soybean. Four to six times the number of bacteria from antagonistic plants, compared to soybean, significantly reduced disease incidence of both nematodes. No single pattern of physiological reactions was common among all these bacteria, suggesting that multiple mechanisms accounted for the observed biological control. The results suggest that rhizospheres of antagonistic plants may be useful sources of potential biological control agents for phytopathogenic nematodes.
引用
收藏
页码:75 / 84
页数:10
相关论文
共 33 条
[1]  
Alam M. M., 1990, Nematode bio-control., P41
[2]  
[Anonymous], RHIZOSPHERE PLANT GR
[3]   EFFECTS OF RHIZOBACTERIA ON ROOT-KNOT NEMATODES AND GALL FORMATION [J].
BECKER, JO ;
ZAVALETAMEJIIA, E ;
COLBERT, SF ;
SCHROTH, MN ;
WEINHOLD, AR ;
HANCOCK, JG ;
VANGUNDY, SD .
PHYTOPATHOLOGY, 1988, 78 (11) :1466-1469
[4]   DIFFERENTIATION OF ERWINIA-CAROTOVORA SSP CAROTOVORA AND ERWINIA-CAROTOVORA SSP ATROSEPTICA ON THE BASIS OF CELLULAR FATTY-ACID COMPOSITION [J].
DEBOER, SH ;
SASSER, M .
CANADIAN JOURNAL OF MICROBIOLOGY, 1986, 32 (10) :796-800
[5]   A ROOT-TISSUE CULTURE SYSTEM TO STUDY WINTER WHEAT-RHIZOBACTERIA INTERACTIONS [J].
DEFREITAS, JR ;
GERMIDA, JJ .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1990, 33 (05) :589-595
[6]  
Dropkin V. H., 1989, INTRO PLANT NEMATOLO
[7]   CHARACTERIZATION OF FATTY-ACID METHYL-ESTER CONTENT OF CLAVIBACTER-MICHIGANENSIS SUBSP MICHIGANENSIS [J].
GITAITIS, RD ;
BEAVER, RW .
PHYTOPATHOLOGY, 1990, 80 (04) :318-321
[8]  
GRANDADOSALVARE.N, 1989, THESIS COLEGIO POSTG
[9]   A SELECTIVE MEDIUM FOR ENUMERATION AND RECOVERY OF PSEUDOMONAS-CEPACIA BIOTYPES FROM SOIL [J].
HAGEDORN, C ;
GOULD, WD ;
BARDINELLI, TR ;
GUSTAVSON, DR .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (09) :2265-2268
[10]  
Hildebrand DC, 1988, LABORATORY GUIDE IDE, P60