CHARACTERIZATION OF ELICITOR-INDUCTION OF SESQUITERPENE CYCLASE ACTIVITY IN POTATO-TUBER TISSUE

被引：14

作者：

ZOOK, MN ^{[1
]}

CHAPPELL, J ^{[1
]}

KUC, JA ^{[1
]}

机构：

[1] UNIV KENTUCKY, DEPT AGRON, LEXINGTON, KY 40546 USA

来源：

PHYTOCHEMISTRY | 1992年 / 31卷 / 10期

关键词：

SOLANUM-TUBEROSUM; SOLANACEAE; BIOSYNTHESIS; SESQUITERPENOIDS; RISHITIN; 5-EPI-ARISTOLOCHENE; SESQUITERPENE CYCLASE;

D O I：

10.1016/0031-9422(92)83703-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The olefin reaction products of the potato and tobacco sesquiterpene cyclases were partially separated from each other by argenation chromatography. Arachidonic acid-elicited potato tuber discs or cellulase-elicited tobacco cell suspension cultures metabolized the olefin reaction products of potato and tobacco sesquiterpene cyclases into different sets of compounds. These results provide evidence that the two olefin cyclase products are chemically different. The K(m) of the potato sesquiterpene cyclase for farnesyl diphosphate was 7 muM and 1.0 mM for magnesium chloride. Inorganic diphosphate inhibited enzyme activity with an ID50 of approximately 4 mM.

引用

页码：3441 / 3445

页数：5

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