SEGMENTATION AND CLASSIFICATION OF BACTERIAL CULTURE IMAGES

被引:26
作者
DUBUISSON, MP
JAIN, AK
JAIN, MK
机构
[1] MICHIGAN STATE UNIV,DEPT ANIM SCI,E LANSING,MI 48824
[2] MICHIGAN BIOTECHNOL INST,LANSING,MI 48909
关键词
METHANOGEN; MICROBIAL ECOLOGY; MICROBIAL IDENTIFICATION; IMAGE PROCESSING; IMAGE SEGMENTATION; PATTERN RECOGNITION;
D O I
10.1016/0167-7012(94)90031-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A procedure using simple image processing and pattern recognition techniques to automatically extract and count organisms using images of bacterial cultures is described in this paper. Methanospirillum hungatei and Methanosarcina mazei were used for the experiments. The methanogens were grown in anaerobic serum vials for 1-3 week period. Samples of the growing cultures were withdrawn from the vials and photographed. The procedure was applied to images containing one kind of organism as well as images containing both types of organisms. The procedure separated and correctly identified the cultures as M. hungatei and M. mazei even in images where the organisms were overlapping or touching. The edge detection, the splitting, and the classification stages, by using this procedure, were accomplished in less than a minute. The image analysis and pattern recognition techniques described in this paper are simple, fast, and able to identify organisms based on their shapes. Our results indicate that such systems can be used to semi-automate routine culture methods allowing rapid direct microscopic techniques for the detection, enumeration, and identification of bacteria.
引用
收藏
页码:279 / 295
页数:17
相关论文
共 18 条
[2]  
EVANSHURRELL JA, 1993, FEMS MICROBIOL LETT, V107, P77
[3]   THE AUTONOMOUS IMAGE ANALYZER - ENUMERATION, MEASUREMENT AND IDENTIFICATION OF MARINE-PHYTOPLANKTON [J].
GORSKY, G ;
GUILBERT, P ;
VALENTA, E .
MARINE ECOLOGY PROGRESS SERIES, 1989, 58 (1-2) :133-142
[4]  
GREEN PE, 1976, MATH TOOLS APPLIED M
[5]  
Haralick R.M., 1993, COMPUTER ROBOT VISIO, V2
[6]   IMAGE SEGMENTATION TECHNIQUES [J].
HARALICK, RM ;
SHAPIRO, LG .
COMPUTER VISION GRAPHICS AND IMAGE PROCESSING, 1985, 29 (01) :100-132
[7]   SEGMENTATION OF X-RAY AND C-SCAN IMAGES OF FIBER REINFORCED COMPOSITE-MATERIALS [J].
JAIN, AK ;
DUBUISSON, MP .
PATTERN RECOGNITION, 1992, 25 (03) :257-270
[8]   SEGMENTATION OF MUSCLE-CELL PICTURES - A PRELIMINARY-STUDY [J].
JAIN, AK ;
SMITH, SP ;
BACKER, E .
IEEE TRANSACTIONS ON PATTERN ANALYSIS AND MACHINE INTELLIGENCE, 1980, 2 (03) :232-242
[9]  
JAIN AK, 1988, INT ENCY ROBOTICS AP, P1052
[10]   INFLUENCE OF CORRINOID ANTAGONISTS ON METHANOGEN METABOLISM [J].
KENEALY, W ;
ZEIKUS, JG .
JOURNAL OF BACTERIOLOGY, 1981, 146 (01) :133-140