UREASE DEFECTIVE MUTANTS IN NEUROSPORA CRASSA

被引：13

作者：

KOLMARK, HG

机构：

[1] Instituto of Physiological Botany, University of Uppsala, Uppsala

来源：

MOLECULAR AND GENERAL GENETICS | 1969年 / 104卷 / 03期

关键词：

D O I：

10.1007/BF02539286

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for isolating urease mutants was developed. It is based on the use of microconidial strains with small and compact colonies. Mutants are detected by their inability to change the color of a pH indicator when they are brought in contact with a solution of urea. The assay is performed in the absence of growth conditions so that the colonies remain separate. Two isolated urease mutants are unable to grow on urea as the sole source of nitrogen, but grow as well as the wild type on other sources of nitrogen. The same two mutants give rise to different acidities in liquid growth medium. The two mutants are also genetically different (Kølmark, 1969 b). The finding that two genetically and physiologically distinct loci participate in the control of one enzyme is discussed. © 1969 Springer-Verlag.

引用

页码：219 / &

共 43 条

[1]

AURICH H., 1959, PHYTON REV INTERNAC BOT EXPTL ARGENTINA, V12, P109

[2] EFFECT OF ENVIRONMENTAL CONDITIONS ON NADP-SPECIFIC GLUTAMIC ACID DEHYDROGENASE IN NEUROSPORA CRASSA [J].