PULSED-FIELD GEL-ELECTROPHORESIS OF DNA FROM 4 MICROSPORIDIAN ISOLATES

被引：25

作者：

MALONE, LA

MCIVOR, CA

机构：

[1] Horticulture and Food Research Institute of New Zealand Ltd., Mt. Albert Research Centre, Auckland

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 1993年 / 61卷 / 02期

关键词：

MICROSPORIDIA; VAVRAIA-ONCOPERAE; NOSEMA-COSTELYTRAE; VAIRIMORPHA SP; COSTELYTRA-ZEALANDICA; PIERIS-RAPAE; WISEANA SPP; PULSED-FIELD GEL ELECTROPHORESIS; CHEF; CHROMOSOMES;

D O I：

10.1006/jipa.1993.1036

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Pulsed-field gel electrophoresis is used to visualize chromosome-sized DNA molecules of four microsporidian isolates from pest insects. Vavraia oncoperae isolated from Wiseana spp. caterpillars had 16 resolvable bands and the same species isolated from grass grubs had 14 resolvable bands. Chromosomal bands were more difficult to resolve for Nosema costelytrae and a Vairimorpha species from cabbage white butterflies, but electrophoresis revealed that these species had a minimum of 8 bands. The microsporidian DNA molecules resolved by this method are estimated to be between 130 and 1930 kilobases in size. Copyright © 1993 Academic Press. All rights reserved.

引用

页码：203 / 205

页数：3

共 15 条

[1] ELECTROPHORETIC KARYOTYPES OF CLINICALLY ISOLATED YEASTS OF CANDIDA-ALBICANS AND C-GLABRATA [J].