MOLECULAR-CLONING OF 2 PIGMENT-DISPERSING HORMONE (PDH) PRECURSORS IN THE BLUE-CRAB CALLINECTES-SAPIDUS REVEALS A NOVEL MEMBER OF THE PDH NEUROPEPTIDE FAMILY

被引：42

作者：

KLEIN, JM ^{[1
]}

MOHRHERR, CJ ^{[1
]}

SLEUTELS, F ^{[1
]}

RIEHM, JP ^{[1
]}

RAO, KR ^{[1
]}

机构：

[1] UNIV W FLORIDA, DEPT CELLULAR & MOLEC BIOL, PENSACOLA, FL 32514 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 205卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.2680

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA library was established from the eyestalk ganglia of the blue crab Callinectes sapidus. Screening resulted in the isolation of a clone (497 bp excluding poly(A) tail) which encodes a beta-PDH previously found in several crustacean species. It displays high sequence similarity with a clone isolated from an eyestalk cDNA library of the shore crab Carcinus maenas, indicating the close phylogenetic relationship of both species. A second clone (414 bp exclusive of the poly(A) tail) encodes a novel beta-PDH analog which displays 400-fold less potency in crab bioassays. Both cDNAs encode open reading frames of 234 bp for the prepropeptides, consisting of signal peptides, PDH-precursor-related peptides, and PDH sequences. (C) 1994 Academic Press, Inc.

引用

页码：410 / 416

页数：7

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