A COUNTERSELECTABLE PACYC184-BASED LACZ-ALPHA-COMPLEMENTING PLASMID VECTOR WITH NOVEL MULTIPLE CLONING SITES - CONSTRUCTION OF CHROMOSOMAL DELETIONS IN KLEBSIELLA-PNEUMONIAE

被引：16

作者：

GEISSLER, S ^{[1
]}

DRUMMOND, M ^{[1
]}

机构：

[1] UNIV SUSSEX,AFRC,IPSR,NFL,BRIGHTON BN1 9RQ,ENGLAND

来源：

GENE | 1993年 / 136卷 / 1-2期

关键词：

REVERSE GENETICS; RECOMBINANT DNA; SACB; NTRBC; NITROGEN CONTROL;

D O I：

10.1016/0378-1119(93)90474-H

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have constructed a series of small, chloramphenicol-resistance-encoding, lacZ alpha-complementing vectors with novel multiple cloning sites, based on the pACYC184 replicon. The sacB gene of Bacillus subtilis, which is lethal to Gram(-) organisms in the presence of sucrose, was cloned into one of these, giving the counterselectable vector pSG335. This was used to substitute a streptomycin-resistance-encoding cassette for the ntr BC genes in the Klebsiella pneumoniae chromosome.

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页码：253 / 255

页数：3

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