PROTEIN-SYNTHESIS IN MOUSE SPERMATOZOA

被引：32

作者：

BRAGG, PW ^{[1
]}

HANDEL, MA ^{[1
]}

机构：

[1] UNIV TENNESSEE,DEPT ZOOL,KNOXVILLE,TN 37916

来源：

BIOLOGY OF REPRODUCTION | 1979年 / 20卷 / 02期

关键词：

D O I：

10.1095/biolreprod20.2.333

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The present study was undertaken to characterize protein synthesis by mature mouse sperm. It was found that these sperm incorporated amino acids linearly over a period of 3 h and that incorporation was inhibited by incubation of sperm at 4° C and by chloramphenicol but not inhibited by cycloheximide. Labeled proteins from control and chloramphenicol-inhibited sperm were displayed on electrophoretic gels, and the labeled protein bands were characterized with respect to molecular weight and degree of inhibition. Autoradiography of the labeled sperm revealed that most grains were localized over the sperm midpiece. These data indicate that the protein synthesis of mouse sperm is mitochondrial in nature.

引用

页码：33 / 337

页数：305

共 12 条

[1]

BACETTI B, 1975, EXP CELL RES, V90, P183

[2]

BUSBY WF, 1974, BIOCHIM BIOPHYS ACTA, V330, P246

[3] ELECTROPHORETIC ANALYSIS OF MAJOR POLYPEPTIDES OF HUMAN ERYTHROCYTE MEMBRANE [J].