A SIMPLE AND EFFICIENT METHOD FOR THE OLIGONUCLEOTIDE-DIRECTED MUTAGENESIS USING PLASMID DNA-TEMPLATE AND PHOSPHOROTHIOATE-MODIFIED NUCLEOTIDE

被引：29

作者：

SUGIMOTO, M

ESAKI, N

TANAKA, H

SODA, K

机构：

来源：

ANALYTICAL BIOCHEMISTRY | 1989年 / 179卷 / 02期

关键词：

D O I：

10.1016/0003-2697(89)90134-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：309 / 311

页数：3

共 9 条

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[4]

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[5] OLIGONUCLEOTIDE-DIRECTED DOUBLE-STRAND BREAK REPAIR IN PLASMIDS OF ESCHERICHIA-COLI - A METHOD FOR SITE-SPECIFIC MUTAGENESIS [J].

MANDECKI, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (19) :7177-7181

[6] OLIGONUCLEOTIDE-DIRECTED MUTAGENESIS USING PLASMID DNA TEMPLATES AND 2 PRIMERS [J].

SCHOLD, M ;

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REYES, AA ;

WALLACE, RB .

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[7]

Tan Z K, 1983, Cold Spring Harb Symp Quant Biol, V47 Pt 1, P383

[8]

TAYLOR JW, 1985, NUCLEIC ACIDS RES, V13, P8765

[9]

WALLACE RB, 1981, NUCLEIC ACIDS RES, V9, P3647

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