ASCORBIC-ACID BIOSENSOR USING ASCORBATE OXIDASE IMMOBILIZED ON ALKYLAMINE GLASS-BEADS

被引：12

作者：

MARQUES, ETA ^{[1
]}

LIMA, JL ^{[1
]}

机构：

[1] UNIV FED PERNAMBUCO,DEPT BIOQUIM,LAB IMUNOPATOL KEIZO ASAMI,LIKA CIDADE UNIV,BR-50730 RECIFE,PE,BRAZIL

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1992年 / 32卷

关键词：

ASCORBIC ACID; BIOSENSOR; OXYGEN ELECTRODE; CUCURBITA-MAXIMA;

D O I：

10.1007/BF02922149

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A biosensor for ascorbic acid based on enzyme kinetics of ascorbate oxidase (E.C.1.10.3.3.) was developed. The enzyme was extracted from Cucurbita maxima, or jerimun and immobilized by covalent bounding, using glutaradehyde as a bifunctional agent, on alkylamine glass beads, with and without enzyme active site protection. A low-cost, home-made oxygen electrode was applied as a transducer. The system has sensitivity from 62.5 up to 500-mu-M of ascorbic acid with satisfactory operation for more than 2 mo.

引用

页码：73 / 78

页数：6

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