PREPARATION OF POLYACRYLAMIDE GEL-FILLED CAPILLARIES FOR CAPILLARY ELECTROPHORESIS

被引：46

作者：

DOLNIK, V ^{[1
]}

COBB, KA ^{[1
]}

NOVOTNY, M ^{[1
]}

机构：

[1] INDIANA UNIV,DEPT CHEM,BLOOMINGTON,IN 47405

来源：

JOURNAL OF MICROCOLUMN SEPARATIONS | 1991年 / 3卷 / 02期

关键词：

CAPILLARY GEL ELECTROPHORESIS; POLYACRYLAMIDE GEL; ISOTACHOPHORETIC POLYMERIZATION;

D O I：

10.1002/mcs.1220030209

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A procedure for preparing polyacrylamide gel-filled capillaries, for use in capillary gel electrophoresis, is described. The method involves the principle of isotachophoretic polymerization, in which the polymerization initiator is introduced by electromigration to promote gradual polymerization of the acrylamide in a sequential manner along the length of the capillary. This procedure has been found to be superior to other methods for polymerizing gels inside of small-bore (i.e., 50-mu-m i.d.) capillaries, because of the reduced formation of bubbles in the gels during polymerization. The method described is simple, reproducible, and several capillaries can be prepared simultaneously. A preliminary result showing the use of 50-mu-m i.d. gel-filled capillaries for SDS-polyacrylamide gel electrophoresis of proteins is shown.

引用

页码：155 / 159

页数：5

共 18 条

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