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IDENTIFICATION, PURIFICATION AND CHARACTERIZATION OF A NOVEL PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C, A 3RD MEMBER OF THE BETA-SUBFAMILY

被引：27
作者：
CAROZZI, AJ
KRIZ, RW
WEBSTER, C
PARKER, PJ
机构：
[1] IMPERIAL CANC RES FUND,POB 123,LINCOLNS INN FIELDS,LONDON WC2A 3PX,ENGLAND
[2] GENET INST,CAMBRIDGE,MA
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1992年 / 210卷 / 02期
关键词：
D O I：
10.1111/j.1432-1033.1992.tb17450.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The partial sequence of a novel PtdIns-specific phospholipase C of the beta subfamily (Ptdins-PLCbeta3) is described. Based upon the predicted protein sequence, monospecific antibodies have been raised and used to identify a suitable source for purification of the protein. Fractionation of HeLa S3 cells revealed that immunoreactive Ptdins-PLCbeta3 is membrane associated; purification (almost-equal-to 1000-fold) from this fraction yielded a single immunoreactive protein of 158 kDa, with a specific activity of 136 mumol . min-1 . mg-1, with Ptdins 4,5-bisphosphate as substrate. Substrate specificity and Ca2+ dependence of this purified PtdIns-PLC are characteristic of the PtdIns-PLCbeta subfamily.
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页码：521 / 529
页数：9
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